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L-脯氨酸-海藻糖天然深共熔溶剂作为速冻猪肉饼的功能性冷冻保护剂:改善口腔加工与消化性

L-proline-trehalose natural deep eutectic solvent as a functional cryoprotectant for quick-frozen pork patties: improving oral processing and digestibility.

基础研究咽喉科IF 10.5Q1

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中文摘要

本研究采用L-脯氨酸和海藻糖配制天然深共熔溶剂(NADES),评估其对速冻猪肉饼冰晶行为、吞咽适宜性及体外消化性的调控作用。结果表明,20% H₂O NADES可降低黏度,同时保持强氢键。冷冻180天后,4% NADES组的冰晶含量最低(32.38%)。口腔加工评估显示,NADES组的摩擦系数显著低于对照组(P < 0.05),有效维持了速冻猪肉饼的口腔润滑性。SDS-PAGE和CLSM证实,NADES可抑制冷冻诱导的蛋白质聚集,并生成更多低分子量肽,从而促进消化。总之,NADES作为一种绿色抗冻剂,可改善冷冻肉制品的品质,并为开发易于吞咽和消化的老年人肉制品提供了新途径。

英文摘要

This study used L-proline and trehalose to formulate a natural deep eutectic solvent (NADES). It evaluated its regulatory effects on the ice crystal behavior, swallowing suitability, and in vitro digestibility of quick-frozen pork patties. The results show that 20% H₂O NADES can reduce viscosity while maintaining strong hydrogen bonds. After 180 days of freezing, the 4% NADES group had the lowest ice crystal content (32.38%). An oral processing assessment revealed that the NADES group exhibited a significantly lower coefficient of friction than the control group (P < 0.05), effectively maintaining the oral lubricity of quick-frozen pork patties. SDS-PAGE and CLSM confirmed that NADES inhibits freezing-induced protein aggregation and generates more low-molecular-weight peptides, thereby promoting digestion. In summary, NADES, as a green anti-freezing agent, can improve the quality of frozen meat products and offers a new approach to developing meat products for the elderly that are easy to swallow and digest.