过敏性鼻炎对重度腺样体肥大儿童鼻咽部微生物群及代谢组学的影响

文献信息

中文摘要

引言： 过敏性鼻炎（AR）对儿童腺样体肥大（AH）的影响尚不明确。本研究旨在探讨AR对AH儿童鼻咽部微生物群及代谢组学的影响。
方法： 采用16S rDNA测序和非靶向代谢组学评估9例AH合并AR儿童（AH-AR组）和9例AH未合并AR儿童（AH-nAR组）的鼻咽部微生物群及代谢组学特征。
结果： 16S rDNA测序结果显示，与AH-nAR组相比，AH-AR组鼻咽部微生物群多样性显著增加，主要表现为梭杆菌属、放线菌属和韦荣球菌属显著增加。进一步代谢组学显示，与AH-nAR组相比，AH-AR组腺样体表面分泌物样本中有140种代谢物显著减少、115种代谢物显著增加。KEGG富集分析显示，AH-AR组有12条代谢通路发生显著变化。此外，Spearman相关分析显示，发生改变的细菌属与代谢物之间存在显著相关性。
讨论： AR可能通过鼻咽部微生物群及代谢物促进儿童AH。

英文摘要

INTRODUCTION: The impact of allergic rhinitis (AR) on adenoid hypertrophy (AH) in pediatric patients is unclear. This study aims to investigate the effects of AR on the nasopharyngeal microbiota and metabolomics of children with AH.
METHODS: 16S rDNA sequencing and untargeted metabolomics were used to evaluate the nasopharyngeal microbiota and metabolomics characteristics of 9 children with AH complicated with AR (AH-AR group) and 9 children with AH without complicated AR (AH-nAR group).
RESULTS: The 16S rDNA sequencing results showed that compared with the AH-nAR group, the diversity of nasopharyngeal microbiota in the AH-AR group significantly increased, mainly manifested by a significant increase in Fusobacterium, Actinomyces, and Veillonella genera. Further metabolomics showed that compared with the AHnAR group, 140 metabolites were significantly reduced and 115 metabolites were significantly increased in the adenoid surface secretion samples of the AH-AR group. KEGG enrichment analysis showed significant changes in 12 metabolic pathways in the AH-AR group. In addition, Spearman correlation analysis revealed a significant correlation between altered bacterial genera and metabolites.
DISCUSSION: AR may promote AH in children through the nasopharyngeal microbiota and metabolites.