NCCRP1表达与头颈部鳞状细胞癌FaDu细胞T分期及体外生长相关特征的关联

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中文摘要

非特异性细胞毒性细胞受体蛋白1（NCCRP1）表达于正常鳞状上皮，已被报道为食管鳞状细胞癌和三阴性乳腺癌的预后标志物。在此，我们评估了NCCRP1在头颈部鳞状细胞癌（HNSCC）及邻近正常黏膜中的表达、其与临床病理特征的关联，以及强制表达NCCRP1后的体外生长相关特征。在本研究中，我们评估了43例HNSCC患者经活检和切除采集的福尔马林固定、石蜡包埋标本中NCCRP1的表达水平，并检查了NCCRP1表达与临床病理特征的关联。使用MTT法和集落形成实验评估强制表达NCCRP1的人HNSCC FaDu细胞。我们发现NCCRP1表达于口腔、鼻咽、中咽、下咽和喉的非癌性棘层和浅表上皮层。HNSCC组织中NCCRP1表达显示出相当大的变异，与周围非癌黏膜中相对均匀的表达形成对比。根据肿瘤中NCCRP1染色比例，43例患者中31例（72.1%）被分类为NCCRP1低表达，其余12例（27.9%）患者为NCCRP1高表达。HNSCC组织中NCCRP1表达与T分期显示出探索性关联（p = 0.0215）。强制表达NCCRP1的HNSCC FaDu细胞通过MTT和集落形成实验测量显示出增强的体外生长相关特征。在该选定的HNSCC队列中，NCCRP1表达与T分期相关，并且FaDu细胞中强制NCCRP1过表达与体外生长相关测量增加相关。然而，这些探索性发现需要在独立临床队列和实验模型中得到验证。

英文摘要

Nonspecific cytotoxic cell receptor protein 1 (NCCRP1), which is expressed in normal squamous epithelium, has been reported as a prognostic marker for esophageal squamous cell carcinoma and triple-negative breast cancer. Here, we evaluated expression of NCCRP1 in head and neck squamous cell carcinoma (HNSCC) and adjacent normal mucosa, its association with clinicopathological features, and in vitro growth-related characteristics following forced NCCRP1 expression. In the present study, we evaluated expression levels of NCCRP1 in formalin-fixed, paraffin-embedded samples of specimens collected by biopsy and resection from 43 patients with HNSCC and examined association of NCCRP1 expression with clinicopathological characteristics. Human HNSCC FaDu cells with forced NCCRP1 expression were assessed using the MTT assay and the colony formation assay. We found that NCCRP1 was expressed in noncancerous spinosum and superficial epithelial layers of the oral cavity, nasopharynx, mesopharynx, hypopharynx, and larynx. NCCRP1 expression in HNSCC tissues showed considerable variation, contrasting to relatively uniform expression in noncancerous surrounding mucosa. Based on NCCRP1 staining proportion in tumor, 31 of the 43 patients (72.1%) were classified as NCCRP1-low and the remaining 12 (27.9%) patients as NCCRP1-high. NCCRP1 expression in HNSCC tissue showed an exploratory association with T category (p = 0.0215). HNSCC FaDu cells with forced NCCRP1 expression exhibited enhanced in vitro growth-related characteristics measured by the MTT and colony formation assays. NCCRP1 expression was associated with T category in this selected HNSCC cohort, and forced NCCRP1 overexpression in FaDu cells was associated with increased in vitro growth-related measurements. Nevertheless, these exploratory findings require validation in independent clinical cohorts and experimental models.