配对活检表面与组织微生物组分析揭示鼻咽癌中组织相关细菌特征

文献信息

中文摘要

引言： 肿瘤相关微生物群已被认为与癌症生物学有关，但活检表面检测到的细菌与肿瘤组织内检测到的细菌之间的关系仍不明确。
方法： 在本研究中，我们采用五区域16S rRNA基因测序，对鼻咽癌（NPC）患者和良性鼻咽淋巴增生（NLH）对照者的配对活检表面拭子和组织活检进行了分析。对匹配的bulk RNA-seq数据进行分析，以探索微生物特征、Epstein-Barr病毒（EBV）转录、宿主通路活性以及推断的免疫细胞组成之间的关联。
结果： 在该发现队列中，组织相关微生物谱在NPC与NLH之间的分离程度强于活检表面微生物谱。若干在NPC中富集的种水平分类学归属与较高的EBV转录水平、增殖和细胞周期通路活性以及较低的推断CD8 T细胞和浆细胞样树突状细胞特征相关。
讨论： 这些发现表明，NPC组织含有与活检表面群落不同的微生物特征，并支持进一步验证组织相关微生物-EBV-宿主之间的关系。

英文摘要

INTRODUCTION: Tumor-associated microbiota have been implicated in cancer biology, but the relationship between bacteria detected on the biopsy surface and those detected within tumor tissue remains poorly defined.
METHODS: In this study, we profiled paired biopsy-surface swabs and tissue biopsies from patients with nasopharyngeal carcinoma (NPC) and benign nasopharyngeal lymphoid hyperplasia (NLH) controls using five-region 16S rRNA gene sequencing. Matched bulk RNA-seq data were analyzed to explore associations between microbial features, Epstein-Barr virus (EBV) transcription, host pathway activity, and inferred immune-cell composition.
RESULTS: Tissue-associated microbial profiles showed stronger separation between NPC and NLH than biopsy-surface profiles in this discovery cohort. Several NPC-enriched species-level taxonomic assignments were associated with higher EBV transcript levels, proliferative and cell-cycle pathway activity, and lower inferred CD8 T-cell and plasmacytoid dendritic-cell signatures.
DISCUSSION: These findings indicate that NPC tissue contains microbial signatures that differ from biopsy-surface communities and support further validation of tissue-associated microbe-EBV-host relationships.