Article record

用于在严重前庭损伤小鼠模型中判定VOR存在的非侵入性视频眼动图装置的开发

Development of a noninvasive video-oculography setup for determining VOR presence in a severe vestibular impairment mouse model.

基础研究耳科IF 3.2Q1

文献信息

PMID	42950697
原文	在 PubMed 查看原文 ↗
发表日期	2026
作者	Fien Aben 查看其他 8 位作者 Lien Bossaerts；Geert Keteleer；Aïda Palou；Ana Renner；Jordi Llorens；Krystyna Szewczyk；Dorien Verdoodt；Vincent Van Rompaey
作者单位	Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: fien.aben@uantwerpen.be. 查看其他 8 条单位信息 Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: lienbossaerts@hotmail.com.；Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: geert.keteleer@uantwerpen.be.；Departament de Ciències Fisiològiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociènces, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardó, Barcelona, 08035, Spain; Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: apaloumi45@ub.edu.；Departament de Ciències Fisiològiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociènces, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardó, Barcelona, 08035, Spain; Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: ana.renner@ub.edu.；Departament de Ciències Fisiològiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociènces, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardó, Barcelona, 08035, Spain; Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: jllorens@ub.edu.；Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: krystyna.szewczyk@uantwerpen.be.；Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium; Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: Dorien.Verdoodt@uantwerpen.be.；Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium; Department of Otorhinolaryngology and Head & Neck Surgery, Antwerp University Hospital, Drie Eikenstraat 655, Edegem, 2650, Belgium. Electronic address: vincent.vanrompaey@uantwerpen.be.
期刊	Hearing research
SCI 分区	Q1
IF	3.2
研究类型	基础研究 · 基础／转化
所属专科	耳科

中文摘要

前庭眼反射（VOR）在头部运动时稳定凝视，是前庭完整性的关键指标。传统的小鼠VOR测量系统通常需要侵入性头部固定，对动物福利和实验可重复性构成风险。在本研究中，我们开发并验证了一种非侵入性固定装置，用于在严重前庭损伤小鼠模型中检测VOR反应的存在，为手术固定系统提供了一种以福利为导向的替代方案。我们使用视频眼动图（VOG）评估了对照组和前庭损伤CD-1小鼠的VOR反应，前庭功能障碍通过口服耳毒性化合物烯丙基腈诱导。正如预期，烯丙基腈处理的小鼠表现出VOR反应几乎完全缺失，证实了严重的前庭损伤。补充评估包括前庭功能障碍指数（VDI）评分、游泳测试、前庭嵴毛细胞计数的免疫荧光染色以及听力测试。由于所诱导的严重缺陷使本研究无法进行定量的增益和相位分析，该装置目前仅限于在该模型中进行二分类VOR检测。然而，进一步的研究将确定在部分或进行性损伤条件下，该非侵入性系统是否支持更详细的VOR动力学表征。总体而言，此处展示的优化、无手术装置提高了小鼠前庭测试的可重复性和福利标准，为在严重前庭损伤小鼠模型中检测VOR存在提供了一个改进的平台。

英文摘要

The vestibulo-ocular reflex (VOR), which stabilizes gaze during head movement, serves as a key indicator of vestibular integrity. Conventional systems for VOR measurement in mice often require invasive head fixation, posing risks to animal welfare and experimental reproducibility. In this study, we developed and validated a noninvasive immobilization setup for detecting the presence of VOR responses in a severe vestibular impairment mouse model, offering a welfare-oriented alternative to surgically fixed systems. Using video-oculography (VOG), we assessed VOR responses in control and vestibular-impaired CD-1 mice, with vestibular dysfunction induced via oral administration of the ototoxic compound allylnitrile. As expected, allylnitrile-treated mice exhibited a near-complete absence of VOR responses, confirming profound vestibular impairment. Complementary assessments included Vestibular Dysfunction Index (VDI) scoring, swimming tests, immunofluorescent staining for hair cell counts in vestibular cristae and hearing tests. Because the induced, severe deficits precluded quantitative gain and phase analysis in this study, the set-up is currently limited to binary VOR detection in this model. However, further research will determine if under conditions of partial or progressive impairment, this noninvasive system supports more detailed characterization of VOR dynamics. Overall, the optimized, surgery-free setup demonstrated here enhances the reproducibility and welfare standards of vestibular testing in mice, offering a refined platform for detecting VOR presence in a severe vestibular impairment mouse model.