穴位埋线减轻过敏性鼻炎大鼠的喷嚏行为，并与NMB-NMBR-cVRG信号通路的减弱相关。

文献信息

中文摘要

目的： 探讨迎香穴穴位埋线（ACE）能否减轻过敏性鼻炎（AR）大鼠模型的喷嚏行为，并探究其与NMB-NMBR-cVRG神经通路减弱的关系。
方法： 将84只SPF级SD大鼠初始分为对照组（n = 24）和OVA致敏组（n = 60）。研究分两个连续阶段进行。第一阶段（n = 24），通过将随机抽取的模型大鼠亚组（n = 12）与对照组（n = 12）进行比较，验证AR模型的成功建立。第二阶段，在确认造模成功后，将剩余的AR大鼠（n = 48）随机分为四个治疗亚组（每组n = 12）：AR模型组、ACE组、NMB-siRNA组和NMB-nc组。剩余对照大鼠（n = 12）作为空白组。主要结局指标喷嚏频率和呼吸气流模式通过全身体积描记法（WBP）评估。次要指标包括鼻腔炎症介质（ELISA）、黏膜组织病理学（HE），以及通过蛋白质印迹（WB）、免疫组织化学（IHC）和免疫荧光（IF）测定的三叉神经-脑干轴中NMB、NMBR和c-Fos的蛋白表达。
结果： 1. 通过卵清蛋白致敏成功建立AR模型。2. 与AR模型组相比，ACE组显著减轻喷嚏症状，降低鼻灌洗液中AR相关炎症因子水平，并改善鼻黏膜病理损伤。3. 与AR模型组相比，ACE组和NMB-siRNA组中NMB、NMBR和c-Fos的表达水平均显著降低。
结论： 迎香穴ACE减弱炎症刺激诱导的NMB-NMBR-cVRG通路内神经元激活。这些发现提示ACE在调节AR大鼠中枢喷嚏反射敏感性和神经源性炎症中发挥关键作用。

英文摘要

OBJECTIVE: To investigate whether Yingxiang acupoint catgut embedding (ACE) can alleviate the sneezing behavior in a rat model of allergic rhinitis (AR), and to explore its relationship with the attenuation of the NMB-NMBR-cVRG neural pathway.
METHODS: Eighty-four SPF-grade SD rats were initially assigned to a control group (n = 24) and an OVA-sensitization group (n = 60). The study was conducted in two sequential stages. In the first stage (n = 24), the successful establishment of the AR model was validated by comparing a randomized subset of model rats (n = 12) with the control group (n = 12). In the second stage, following confirmed modeling, the remaining AR rats (n = 48) were randomized into four treatment subgroups (n = 12 each): AR model, ACE, NMB-siRNA, and NMB-nc groups. The remaining control rats (n = 12) served as the blank group. The primary outcome measures, sneezing frequency and respiratory airflow patterns, were evaluated by whole-body plethysmography (WBP). Secondary measures included nasal inflammatory mediators (ELISA), mucosal histopathology (HE), and protein expression of NMB, NMBR, and c-Fos in the trigeminal-brainstem axis determined via Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF).
RESULTS: 1. The AR model was successfully established by ovalbumin sensitization. 2. Compared with the AR model group, the ACE group significantly alleviated sneezing symptoms, reduced the levels of AR-related inflammatory factors in nasal lavage fluid, and improved nasal mucosal pathological damage. 3. Compared with the AR model group, the expression levels of NMB, NMBR, and c-Fos were significantly decreased in both the ACE group and the NMB-siRNA group.
CONCLUSION: ACE at Yingxiang attenuates inflammatory stimulus-induced neuronal activation within the NMB-NMBR-cVRG pathway. These findings suggest that ACE plays a critical role in modulating central sneeze reflex sensitivity and neurogenic inflammation in AR rats.