工程化水纳米结构灭活雪松花粉过敏原并减轻小鼠模型中的过敏性鼻炎

文献信息

中文摘要

本研究将工程化水纳米结构（EWNSs）——含活性氧的电 charged 水纳米颗粒——确定为一种有效的非药物工具，用于中和日本雪松花粉过敏原。通过将花粉及其主要过敏原 Cry j 1 暴露于 EWNSs，研究人员观察到过敏原性在 24 小时内显著降低，有效阻止树突状细胞活化并抑制 IL-6 和 TNF-α 等促炎细胞因子。在 BALB/c 小鼠中的体内试验表明，EWNS 处理显著降低 IgE 水平，抑制 2 型固有淋巴细胞（ILC2）扩增，并减轻嗜酸性气道炎症。此外，7 天的 EWNS 吸入与更高的外周血氧饱和度和更低的 Th2 细胞因子表达相关，在测量的终点中未观察到不良效应。这些发现支持 EWNS 预处理降低雪松花粉制剂免疫反应性的潜力，并表明在所测试条件下 EWNS 吸入具有短期抗炎关联。空气传播花粉的灭活和全面吸入安全性仍有待确定。

英文摘要

This study identifies Engineered Water Nanostructures (EWNSs)-electrically charged water nanoparticles containing reactive oxygen species-as a potent non-pharmacological tool for neutralizing Japanese cedar pollen allergens. By exposing pollen and its primary allergen, Cry j 1, to EWNSs, researchers observed a significant reduction in allergenicity within 24 h, effectively preventing dendritic cell activation and suppressing pro-inflammatory cytokines like IL-6 and TNF-α. In vivo tests on BALB/c mice demonstrated that EWNS treatment significantly lowered IgE levels, inhibited the expansion of Type 2 innate lymphoid cells (ILC2), and attenuated eosinophilic airway inflammation. Furthermore, seven days of EWNS inhalation was associated with higher peripheral oxygen saturation and lower Th2 cytokine expression, without adverse effects in the measured endpoints. These findings support the potential of EWNS pretreatment to lower the immunoreactivity of cedar pollen preparations and indicate short-term anti-inflammatory associations with EWNS inhalation under the conditions tested. Airborne pollen inactivation and comprehensive inhalation safety remain to be established.