CpG佐剂化卵内重组HVT-LT和FPV-LT疫苗对14日龄肉鸡传染性喉气管炎攻毒挑战的免疫原性与效力
Immunogenicity and Efficacy of CpG-Adjuvanted In-Ovo Recombinant HVT-LT and FPV-LT Vaccines Against Infectious Laryngotracheitis Challenge in 14-Day-Old Broiler Chickens.
文献信息
| PMID | 42944016 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Carissa Gaghan |
| 作者单位 | Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, 1060 William Moore Drive, Raleigh, NC 27606, USA. |
| 期刊 | Viruses |
| SCI 分区 | Q2 |
| IF | 3.8 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 咽喉科 |
中文摘要
背景： 重组火鸡疱疹病毒喉气管炎（rHVT-LT）和禽痘病毒（rFPV）-LT卵内疫苗用于肉鸡ILT控制。此前，我们已表明rHVT-LT的卵内CpG-寡脱氧核苷酸（CpG-ODN）佐剂化可在28日龄保护肉鸡抵抗ILT。在此，我们评估了卵内CpG-ODN佐剂化rHVT-LT和rFPV-LT在14日龄攻毒时对肉鸡的免疫原性和保护效力。方法：十八个实验组包括仅CpG-ODN处理组，或卵内接受rHVT-LT或rFPV-LT联合/不联合CpG-ODN或非CpG对照ODN的组，随后在14日龄进行ILTV攻毒。结果：虽然没有任何疫苗接种组提供显著的临床保护，但与未接种对照相比，rHVT-LT+CpG-ODN疫苗接种在ILTV攻毒后显示疾病严重程度的数值降低（p = 0.0710）。仅CpG-ODN或CpG-ODN佐剂化疫苗接种后攻毒导致气管和/或脾脏TLR21、IL-12或IFNγ转录增加（p < 0.05），表明TLR介导的抗病毒佐剂效应。此外，接受ILTV的CpG-ODN+rHVT-LT/rFPV-LT组脾脏巨噬细胞、γδ T细胞、B细胞和活化CD4+/CD8+ T细胞频率增加（p < 0.05）。结论：总体而言，我们的发现表明，卵内CpG-ODN佐剂化联合重组载体ILT疫苗可在肉鸡早期ILTV攻毒后诱导可测量的免疫激活和ILT疾病严重程度降低的趋势。
英文摘要
Recombinant herpesvirus of turkey laryngotracheitis (rHVT-LT) and fowlpox virus (rFPV)-LT in-ovoin-ovo vaccines are used for ILT control in broiler chickens. Previously, we showed that in-ovoin-ovo CpG-oligodeoxynucleotide (CpG-ODN) adjuvantation of rHVT-LT can protect broilers against ILT at 28 days of age. Here, we evaluated the immunogenicity and protective efficacy of in-ovoin-ovo CpG-ODN-adjuvanted rHVT-LT and rFPV-LT in broilers when challenged at 14 days of age. Eighteen experimental groups incorporated CpG-ODN-only treatments or those receiving rHVT-LT or rFPV-LT with/without CpG-ODN or non-CpG control ODN in-ovoin-ovo, followed by ILTV challenge at 14 days of age. While none of the vaccinated groups provided significant clinical protection, rHVT-LT+CpG-ODN vaccination showed a numerical reduction (p = 0.0710) in disease severity following ILTV challenge compared with the unvaccinated control. CpG-ODN-only or CpG-ODN-adjuvanted vaccinations followed by challenge resulted in increased (p < 0.05) tracheal and/or splenic TLR21, IL-12, or IFNγ transcription, indicating TLR-mediated antiviral adjuvant effects. Furthermore, CpG-ODN+rHVT-LT/rFPV-LT groups receiving ILTV had increased (p < 0.05) splenic frequencies of macrophages, γδ T cells, B cells, and activated CD4+/CD8+ T cells. Collectively, our findings indicate that in-ovo CpG-ODN adjuvantation with recombinant-vectored ILT vaccines can induce measurable immune activation and a trend toward reduced ILT disease severity following early ILTV challenge in broiler chickens.