中耳胆脂瘤患者中SIRT1-7 mRNA的表达

文献信息

中文摘要

背景： 中耳胆脂瘤是一种局部破坏性病变，可导致严重并发症，且仅靠药物治疗无法有效控制。尽管sirtuins是细胞代谢、氧化应激和炎症反应的关键表观遗传调控因子，但其在中耳胆脂瘤中的具体作用尚未被探索。本研究旨在探讨sirtuins的表达谱及其与胆脂瘤发病机制和临床特征的关系。
方法： 采用实时定量PCR评估60例接受胆脂瘤手术患者的胆脂瘤组织及配对的耳后皮肤对照中七种sirtuin亚型（SIRT1-7）的相对mRNA表达水平。根据组织类型、患者性别和手术频率（初次与修正）分析表达差异。
结果： 临床上，58.3%的患者观察到耳漏，而细菌培养阳性率为16.7%。传导性听力损失是主要的听力学表现。所有胆脂瘤组织中均检测到SIRT1-7 mRNA表达，其中SIRT1相对表达最高，其次为SIRT4、SIRT3、SIRT6、SIRT7、SIRT5和SIRT2。值得注意的是，与耳后皮肤对照相比，仅SIRT7在胆脂瘤组织中的表达显著下调（p = 0.002）。根据患者性别或修正手术史，SIRT1-7表达未见显著差异（p > 0.05）。
结论： SIRT1-7的独特表达谱表明它们可能参与中耳胆脂瘤的发病机制。尽管胆脂瘤组织中SIRT7 mRNA表达低于耳后皮肤，但这一探索性发现需要使用组织适宜的对照和功能研究进行验证。

英文摘要

Background: Middle ear cholesteatoma is a locally destructive lesion that can cause severe complications and cannot be effectively managed with medical treatment alone. Although sirtuins are crucial epigenetic regulators of cellular metabolism, oxidative stress, and inflammatory responses, their specific roles in middle ear cholesteatoma remain unexplored. This study aimed to investigate the expression profiles of sirtuins and their association with the pathogenesis and clinical characteristics of cholesteatoma. Methods: The relative mRNA expression levels of seven sirtuin isoforms (SIRT1-7) were evaluated using quantitative real-time PCR in cholesteatoma tissues and matched postauricular skin controls obtained from 60 patients undergoing cholesteatoma surgery. Expression differences were analyzed according to tissue type, patient sex, and frequency of surgery (initial versus revision). Results: Clinically, otorrhea was observed in 58.3% of the patients, whereas the bacterial culture positivity rate was 16.7%. Conductive hearing loss was the predominant audiologic configuration. SIRT1-7 mRNA expression was detected in all cholesteatoma tissues, with SIRT1 exhibiting the highest relative expression, followed by SIRT4, SIRT3, SIRT6, SIRT7, SIRT5, and SIRT2. Notably, only the expression of SIRT7 was significantly downregulated in cholesteatoma tissues compared to postauricular skin controls (p = 0.002). No significant differences in SIRT1-7 expression were observed according to patient sex or history of revision surgery (p > 0.05). Conclusions: The distinct expression profiles of SIRT1-7 indicate their potential involvement in the pathogenesis of middle ear cholesteatoma. Although SIRT7 mRNA expression was lower in cholesteatoma tissue than in postauricular skin, this exploratory finding requires validation using tissue-appropriate controls and functional studies.