以儿童为主的队列中过敏表型相关的表观遗传淋巴细胞亚群关联
Epigenetic Lymphocyte Subset Associations Across Allergic Phenotypes in a Predominantly Pediatric Cohort.
文献信息
| PMID | 42938271 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Ergun Mete |
| 作者单位 | Department of Medical Microbiology, Faculty of Medicine, Pamukkale University, 20160 Denizli, Türkiye. |
| 期刊 | Diagnostics (Basel, Switzerland) |
| SCI 分区 | Q1 |
| IF | 3.6 |
| 研究类型 | 临床研究 · 临床 |
| 所属专科 | 鼻科 |
中文摘要
背景/目的： 基于DNA甲基化的淋巴细胞计数可能为传统免疫表型分析提供替代方法，但其与过敏表型的关联需要谨慎解读。我们在以儿童为主的队列中考察了这些关联。方法：这项回顾性研究纳入206名年龄1-57岁的参与者（189名年龄<18岁；57.8%为男性；中位年龄5岁，IQR 3-8），评估时间为2025年11月24日至2026年2月28日。绝对亚群计数由表观遗传i.Mune TBNK检测和CBC淋巴细胞计数得出。组间比较使用Mann-Whitney U检验，并在56次比较中采用Benjamini-Hochberg校正。Firth模型校正了三个亚群计数、CD4/CD8比值、log(IgE+1)、年龄和性别；另加入基于百分比的、合并症校正的和年龄限制的敏感性分析。含IgE的模型包括201例完整病例。结果：过敏性鼻炎在主要模型中与较低的CD4+计数相关（每100个细胞/µL的OR 0.923；p = 0.0009），该关联在合并症校正和年龄限制后仍保留，但在百分比模型中未保留。特应性皮炎与log(IgE+1)相关（OR 1.475；p = 0.0009）。食物过敏在主要模型中与较高的CD19+计数相关（每100个细胞/µL的OR 1.060；p = 0.0341），但该关联在敏感性分析中未保留。食物过敏未发现绝对NK计数差异。单标志物区分能力有限（CD4+对鼻炎的AUC为0.711）。结论：这些探索性关联对测量尺度和模型设定敏感。它们不能确立表型特异性的表观遗传机制、独立的诊断效用或功能性免疫监测。
英文摘要
Background/Objectives: DNA methylation-based lymphocyte enumeration may provide an alternative to conventional immunophenotyping, but its associations with allergic phenotypes require careful interpretation. We examined these associations in a predominantly pediatric cohort. Methods: This retrospective study included 206 participants aged 1-57 years (189 aged <18 years; 57.8% male; median age 5 years, IQR 3-8), evaluated between 24 November 2025 and 28 February 2026. Absolute subset counts were derived from the epigenetic i.Mune TBNK assay and CBC lymphocyte counts. Group comparisons used Mann-Whitney U tests with Benjamini-Hochberg correction across 56 comparisons. Firth models adjusted for three subset counts, CD4/CD8 ratio, log(IgE+1), age, and sex; percentage-based, comorbidity-adjusted, and age-restricted sensitivity analyses were added. IgE-containing models included 201 complete cases. Results: Allergic rhinitis was associated with lower CD4+ counts in the primary model (OR per 100 cells/µL 0.923; p = 0.0009), with the association retained after comorbidity adjustment and age restriction but not in the percentage model. Atopic dermatitis was associated with log(IgE+1) (OR 1.475; p = 0.0009). Food allergy was associated with higher CD19+ counts in the primary model (OR per 100 cells/µL 1.060; p = 0.0341), but this association was not retained in sensitivity analyses. No absolute NK-count difference was found for food allergy. Single-marker discrimination was modest (CD4+ AUC for rhinitis 0.711). Conclusions: These exploratory associations are sensitive to the measurement scale and model specification. They do not establish phenotype-specific epigenetic mechanisms, standalone diagnostic utility, or functional immune monitoring.