Article record

Mesna在中耳手术中安全吗？一项动物研究。

Is Mesna safe in middle ear surgeries? An animal study.

基础研究耳科IF 1.7Q2

文献信息

PMID	42925665
原文	在 PubMed 查看原文 ↗
发表日期	2026
作者	Inabat Yermesheva 查看其他 9 位作者 Ostap Orishchak；Hady Tall；Karina Theoret；Kevork H Atamian；Marie-France Lusignan；Don Nguyen；Danah Quttaineh；Emily Kay-Rivest；Sam J Daniel
作者单位	McGill Otolaryngology Sciences Laboratory, Department of Pediatric Surgery, McGill University, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada. 查看其他 8 条单位信息 McGill Otolaryngology Sciences Laboratory, Department of Pediatric Surgery, McGill University, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada; Division of Pediatric Otolaryngology, Montreal Children's Hospital, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada.；Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, H3G 2M1, Canada.；School of Communication Sciences and Disorders, Dalhousie University, Halifax, NS, Canada.；McGill Otolaryngology Sciences Laboratory, Department of Pediatric Surgery, McGill University, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada; Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, H3G 2M1, Canada.；The Research Institute of the McGill University Health Centre, Montreal, QC, H4A 3J1, Canada.；McGill Otolaryngology Sciences Laboratory, Department of Pediatric Surgery, McGill University, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada; Department of Otolaryngology-Head and Neck Surgery, McGill University Health Centre, Montreal, QC, Canada.；Department of Otolaryngology-Head and Neck Surgery, McGill University Health Centre, Montreal, QC, Canada.；Faculty of Medicine and Health Sciences, McGill University, Montreal, QC, H3G 2M1, Canada; Division of Pediatric Otolaryngology, Montreal Children's Hospital, McGill University Health Centre, Montreal, QC, H4A 3J1, Canada. Electronic address: sam.j.daniel@mcgill.ca.
期刊	International journal of pediatric otorhinolaryngology
SCI 分区	Q2
IF	1.7
研究类型	基础研究 · 基础／转化
所属专科	耳科

中文摘要

目的： Mesna是一种含硫醇化合物，通过其黏液溶解特性分解病理组织，作为化学剥离剂，协助胆脂瘤、鼓室硬化、粘连性中耳炎和鼓膜不张耳等耳科手术。本研究旨在利用动物模型评估100%局部应用Mesna在中耳的耳毒性潜力。
方法： 研究纳入16只雌性白化豚鼠，最终对12只动物进行分析。豚鼠被随机分为两组。第1组——6只豚鼠一只耳接受100% Mesna粉末，另一只耳接受粉末状硼酸作为非耳毒性对照物质。第2组——6只豚鼠一只耳接受100% Mesna溶液，另一只耳接受生理盐水作为非耳毒性对照物质。通过基线（鼓膜切开术后）和给药后4周的听性脑干反应（ABR）测试以及给药后约2个月的耳蜗毛细胞扫描电子显微镜（SEM）检查评估耳毒性。
结果： 任何耳道均未观察到炎症。ABR测量在基线与给药后4周之间保持一致。所有组的SEM分析显示内毛细胞和外毛细胞均无损伤。
结论： 本研究表明，在豚鼠模型中，局部应用100% Mesna（粉末和溶液两种形式）均不诱导耳毒性效应。这些发现支持Mesna用于中耳的安全性，并可能促进其在耳科手术实践中的更广泛采用。

英文摘要

OBJECTIVE: Mesna, a thiol-containing compound, assists in otological surgeries such as cholesteatoma and tympanosclerosis, adhesive otitis media and atelectatic ears by breaking down pathological tissues through its mucolytic properties, acting as a chemical dissector. In this study, the objective was to evaluate the ototoxic potential of 100% topical Mesna administration in the middle ear using an animal model.
METHODS: Sixteen female albino guinea pigs were enrolled in the study. Final analysis was performed on twelve animals. The guinea pigs were randomly divided into two groups. Group 1 - six guinea pigs received 100% Mesna powder in one ear, while the other received powdered boric acid, a non-ototoxic control substance. Group 2 - six guinea pigs received 100% Mesna solution in one ear, while the other received saline solution, as a non-ototoxic control substance. Ototoxicity was assessed through auditory brainstem response (ABR) testing at baseline (postmyringotomy) and 4 weeks post-application and scanning electron microscopy (SEM) of cochlear hair cells approximately 2 months post-application.
RESULTS: No inflammation was observed in any ear canal. ABR measurements remained consistent between baseline and 4 weeks post-application. SEM analysis in all groups revealed no damage to inner or outer hair cells.
CONCLUSION: This study demonstrates that topical application of 100% Mesna, in both powder and solution forms, does not induce ototoxic effects in a guinea pig model. These findings support the safety of Mesna for middle ear use and may promote its broader adoption in otologic surgical practice.