链条上的破洞：同步辐射显微CT揭示小鼠中耳炎中镫骨足板为通向内耳的通道
A hole in the chain: Synchrotron MicroCT reveals a stapes footplate gateway to the inner ear in murine otitis media.
文献信息
| PMID | 42913317 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Diana Cobas |
| 作者单位 | Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA; Center for Neurodegenerative Disease and Therapeutics, Rosalind Franklin University of Medicine and Science, North Chicago, IL, USA; Department of Otolaryngology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. |
| 期刊 | Hearing research |
| SCI 分区 | Q1 |
| IF | 3.2 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 耳科 |
中文摘要
背景： 中耳炎（OM）可通过听骨损伤和耳蜗损伤导致传导性和感音神经性听力损失。然而，其结构随时间的变化过程仍表征不足，且啮齿动物听力研究很少评估中耳疾病，尽管其可能混淆感音神经性听力损失的评估。
目的： 利用同步辐射显微计算机断层扫描（microCT）表征急性和慢性OM中的听骨链变化，并确定感染从中耳向内耳扩散的途径。
方法： 通过鼻内接种假欣茨鲍特菌在小鼠中诱导双侧OM；对照组接受鼻内磷酸盐缓冲盐水（PBS）。在接种后7天（急性OM，N=8）或28天（慢性OM，N=11）评估耳部，并设9只对照耳。颞骨用同步辐射microCT成像（有效像素尺寸：1.625 µm，分辨率：> 3.3 µm），分割并三维重建。在确定标志点评估听骨的侵蚀、穿孔、脱位或骨折。组间差异采用聚类置换检验比较，p值采用Benjamini-Hochberg错误发现率（FDR）程序校正。
结果： 所有OM组均出现结构异常，以砧骨和镫骨受累最重。观察到砧骨体和短突以及锤骨突的异常。镫骨足板侵蚀在对照组中缺失，但在此处研究的所有急性OM耳（8只）中均可见，至慢性时间点降至27.3%（3/11）。
结论： 镫骨足板从急性时间点起即易发生全层侵蚀。该部位可能在人类中藏匿持续性生物膜相关疾病，并且是啮齿动物中耳健康听力研究中的重要混杂变量。未来研究应检查液体或物质通过足板缺损进入内耳的通道。
英文摘要
BACKGROUND: Otitis media (OM) can cause conductive and sensorineural hearing loss through ossicular damage and cochlear injury. Yet the structural time course remains poorly characterized, and rodent hearing studies rarely assess middle ear disease, despite its potential to confound sensorineural hearing loss assessment.
OBJECTIVE: To characterize ossicular chain changes in acute and chronic OM using synchrotron micro-computed tomography (microCT) and identify routes of middle-to-inner-ear spread of infection.
METHODS: Bilateral OM was induced in mice by intranasal inoculation with Bordetella pseudohinzii; controls received intranasal phosphate-buffered saline (PBS). Ears were evaluated at 7 days (acute OM, N= 8) or 28 days (chronic OM, N= 11) post-inoculation, with 9 control ears. Temporal bones were imaged with synchrotron microCT (effective pixel size: 1.625 µm, resolution: > 3.3 µm), segmented, and reconstructed in 3D. Ossicles were assessed for erosion, perforation, dislocation, or fracture at defined landmarks. Group differences were compared using a cluster permutation test, and p-values were adjusted using the Benjamini-Hochberg false discovery rate (FDR) procedure.
RESULTS: Structural abnormalities occurred across all OM groups, with the incus and stapes most affected. Abnormalities of the incus body and short process, as well as the malleolar apophysis, were observed. Stapes footplate erosion was absent in controls but was seen in all the acute-OM ears here investigated (8), decreasing to 27.3% (3/11) at chronic time points.
CONCLUSIONS: The stapes footplate is vulnerable to full-thickness erosion starting at acute time points. This site may harbor persistent biofilm-related disease in humans and is an important confounding variable in rodent hearing research on middle ear health. Future research should examine fluid or substance passage through the footplate defect into the inner ear.