实验性分泌性中耳炎诱导前后益生菌给药：对积液消退和炎症的影响。
Pre- and post-induction probiotic administration in experimental otitis media with effusion: effects on effusion resolution and inflammation.
文献信息
| PMID | 42904746 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Coşkun Ozdemir |
| 作者单位 | Department of Otorhinolaryngology-Head and Neck Surgery, Ministry of Health, Ankara Training and Research Hospital, Ankara, Turkey. |
| 期刊 | Acta oto-laryngologica |
| SCI 分区 | Q3 |
| IF | 1.3 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 耳科 |
中文摘要
背景： 分泌性中耳炎（OME）是一种常见的炎症性中耳疾病，现有药物治疗的疗效仍存在争议。益生菌已成为一种潜在的非抗生素治疗策略。
目的： 比较甲泼尼龙、孟鲁司特和罗伊氏乳杆菌（L. reuteri）在实验诱导的OME模型中的治疗效果。
材料和方法： 将35只雄性Wistar白化大鼠随机分配到五组（n=7）。通过鼓室内注射组胺诱导OME。分组包括对照组、甲泼尼龙组、孟鲁司特组、诱导后益生菌组以及诱导前后益生菌组。通过耳显微镜评估积液消退情况，并通过组织学评估黏膜下炎症性白细胞浸润。
结果： 各组间积液消退情况存在显著差异（p<0.001），甲泼尼龙组达到78.6%，诱导前后益生菌组达到85.7%，而对照组为0%。所有治疗组的黏膜下炎症性白细胞计数均显著低于对照组（p<0.001）。在这项探索性研究中，诱导前后联合益生菌组与甲泼尼龙组之间未检测到炎症性白细胞计数的统计学显著差异。
结论与意义： 罗伊氏乳杆菌在此组胺诱导的OME模型中表现出抗炎活性。与仅诱导后治疗相比，诱导前后联合益生菌给药与更高的积液消退率相关，尽管总暴露时间更长可能对这一发现有所贡献。这些发现支持对益生菌在OME中的进一步研究，但并未确立其与甲泼尼龙等效的疗效。
英文摘要
BACKGROUND: Otitis media with effusion (OME) is a common inflammatory middle ear disease, and the efficacy of available medical treatments remains controversial. Probiotics have emerged as a potential non-antibiotic therapeutic strategy.
AIMS/OBJECTIVES: To compare the efficacy of methylprednisolone, montelukast, and Lactobacillus reuteri (L. reuteri) in the treatment of experimentally induced OME model.
MATERIAL AND METHODS: Thirty-five male Wistar albino rats were randomly assigned to five groups (n = 7). OME was induced by intratympanic histamine injection. Groups comprised control, methylprednisolone, montelukast, post-induction probiotics, and pre- and post-induction probiotics. Effusion resolution was assessed otomicroscopically, and submucosal inflammatory leukocyte infiltration was evaluated histologically.
RESULTS: Effusion resolution differed significantly among groups (p < 0.001), reaching 78.6% with methylprednisolone and 85.7% with pre- and post-induction probiotics, compared with 0% in controls. All treatment groups showed significantly lower submucosal inflammatory leukocyte counts than controls (p < 0.001). No statistically significant difference in inflammatory leukocyte counts was detected between the combined pre- and post-induction probiotic group and the methylprednisolone group in this exploratory study.
CONCLUSIONS AND SIGNIFICANCE: L. reuteri exhibited anti-inflammatory activity in this histamine-induced OME model. Combined pre- and post-induction probiotic administration was associated with greater effusion resolution than post-induction treatment alone, although longer total exposure may have contributed to this finding. These findings support further investigation of probiotics in OME but do not establish equivalent efficacy to methylprednisolone.