外周效应记忆CD4+ T细胞反映儿童过敏性鼻炎的临床症状负担
Peripheral effector-memory CD4+ T cells reflect clinical symptom burden in pediatric allergic rhinitis.
文献信息
| PMID | 42878279 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Takeshi Yamamoto |
| 作者单位 | Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba, Japan. |
| 期刊 | Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology |
| SCI 分区 | Q1 |
| IF | 4.3 |
| 研究类型 | 临床研究 · 临床 |
| 所属专科 | 鼻科 |
中文摘要
背景： 儿童过敏性鼻炎（AR）可显著影响生活质量和学业表现，但传统的致敏标志物如过敏原特异性免疫球蛋白E（sIgE）并不能一致地反映症状严重程度。我们检验了活化诱导标志物（AIM）定义的外周过敏原特异性效应记忆CD4+ T细胞（peff-mCD4）频率是否与症状负担相关。
方法： 在这项单中心观察性初步研究中，分析了20名儿童对屋尘螨（HDM）的特异性反应以及19名儿童对日本柳杉花粉（JCP）的特异性反应。记录了鼻部症状数字评定量表（NRS）和鼻部症状总评分（TNSS）。在短期离体过敏原刺激后，将记忆CD4+ T细胞内的CD154+CD137+细胞定量为peff-mCD4细胞。
结果： 在HDM特异性分析（Spearman ρ分别为0.882和0.835）和JCP特异性分析（ρ分别为0.828和0.769）中，peff-mCD4频率均与NRS和TNSS强相关（所有q < 0.001）。相比之下，sIgE与症状评分的关联较弱，并且在FDR校正后，四项sIgE-症状关联均不再显著。这些peff-mCD4关联在调整年龄和性别后仍然显著。在以TNSS ≥8作为操作阈值的探索性ROC分析中，peff-mCD4对HDM的AUC为0.867，对JCP的AUC为0.910。
结论： peff-mCD4频率与儿童AR的症状负担强相关。需要更大规模的纵向和外部验证研究来确定其作为临床症状负担外周血细胞相关标志物的潜在效用。
英文摘要
BACKGROUND: Pediatric allergic rhinitis (AR) can substantially affect quality of life and school performance, yet conventional sensitization markers such as allergen-specific immunoglobulin E (sIgE) do not consistently reflect symptom severity. We examined whether activation-induced marker (AIM)-defined allergen-specific peripheral effector-memory CD4+ T-cell (peff-mCD4) frequencies were associated with symptom burden.
METHODS: In this pilot, single-center observational study, house dust mite (HDM)-specific responses were analyzed in 20 children and Japanese cedar pollen (JCP)-specific responses in 19. Nasal symptom numerical rating scale (NRS) and total nasal symptom score (TNSS) were recorded. Peff-mCD4 cells were quantified as CD154+CD137+ cells within memory CD4+ T cells after short-term ex vivo allergen stimulation.
RESULTS: Peff-mCD4 frequency was strongly associated with NRS and TNSS in both the HDM-specific (Spearman ρ = 0.882 and 0.835, respectively) and JCP-specific analyses (ρ = 0.828 and 0.769, respectively; all q < 0.001). In contrast, sIgE showed weaker associations with symptom scores, and none of the four sIgE-symptom associations remained significant after FDR adjustment. These peff-mCD4 associations remained significant after adjustment for age and sex. In exploratory ROC analyses using TNSS ≥8 as an operational threshold, peff-mCD4 yielded AUCs of 0.867 for HDM and 0.910 for JCP.
CONCLUSION: Peff-mCD4 frequency was strongly associated with symptom burden in pediatric AR. Larger longitudinal and externally validated studies are required to determine its potential utility as a peripheral blood cellular correlate of clinical symptom burden.