人类男性声带组织层的蛋白质组学分层研究

文献信息

中文摘要

目的： 比较人类真声带被覆层（上皮和浅层固有层；TVFC）、声韧带（中层和深层固有层；VL）及甲杓肌（TA）之间的相对蛋白质组丰度，并表征共有和独特的分子通路。
方法： 采用基于串联质量标签的定量质谱技术，对来自国家组织库的五具人类男性尸体喉组织（平均年龄56.6岁）进行横断面探索性蛋白质组学研究。使用线性混合模型评估差异蛋白丰度，并通过基因本体（GO）富集分析表征各层的功能特化。
结果： 在895种定量蛋白中，554种在三个层中均被检出，181种在TVFC和VL中共同检出，160种仅在TA中检出。差异表达分析显示TVFC与VL之间差异极小（所有FDR > 0.10），但TA与TVFC及VL均呈现显著分离（FDR < 0.05）。GO富集分析表明，TVFC富集于炎症和免疫过程，VL处于中间位置，兼具TVFC和TA的特征，而TA表现出收缩特化。
结论： 这项探索性研究提供了人类声带各层蛋白质表达的初步描述性比较，其中VL作为免疫调节性被覆层与收缩性肌肉之间的潜在过渡区。这些发现证实了这些层之间已确立的组织学、细胞学和功能差异，并为未来喉生物学、病理学和干预研究提供了初步数据。

英文摘要

OBJECTIVES: To compare relative proteomic abundance across the human true vocal fold cover (epithelium and superficial lamina propria; TVFC), vocal ligament (intermediate and deep lamina propria; VL), and thyroarytenoid muscle (TA), and to characterize shared and distinct molecular pathways.
METHODS: Cross-sectional exploratory proteomic study using tandem mass tag-based quantitative mass spectrometry on tissue from five human male cadaveric larynges (mean age 56.6 years) obtained from a national tissue bank. Differential protein abundance was assessed using linear mixed modeling, and Gene Ontology (GO) enrichment analysis characterized functional specialization across layers.
RESULTS: Of 895 quantified proteins, 554 were detected in all three layers, with 181 jointly detected in the TVFC and VL, and 160 detected only in the TA. Differential expression analysis revealed minimal divergence between TVFC and VL (all FDR > 0.10) but robust separation of TA from both the TVFC and VL (FDR < 0.05). GO enrichment demonstrated that TVFC was enriched for inflammatory and immune processes, VL occupied an intermediate position sharing features with both TVFC and TA, and TA demonstrated contractile specialization.
CONCLUSION: This exploratory study provides an initial descriptive comparison of protein expression across human vocal fold layers, with VL serving as a potential transitional zone between the immunomodulatory cover and contractile muscle. These findings confirm the well-established histological, cellular, and functional differences among these layers, and provide preliminary data to inform future studies of laryngeal biology, pathology, and intervention.