生理盐水、链霉素和臭氧鼻腔冲洗对金黄色葡萄球菌诱导的实验性鼻窦炎的比较效果：微生物学、组织病理学和生物膜分析。
Comparative effects of saline, streptomycin, and ozone nasal irrigation on Staphylococcus aureus-induced experimental rhinosinusitis: microbiological, histopathological, and biofilm analyses.
文献信息
| PMID | 42826531 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Gökhan Tüzemen |
| 作者单位 | Ministry of Health, Bursa City Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Bursa, Turkey. |
| 期刊 | Brazilian journal of otorhinolaryngology |
| SCI 分区 | Q2 |
| IF | 2.1 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 鼻科 |
中文摘要
目的： 鼻窦炎以鼻黏膜和鼻旁窦的炎症为特征。鼻腔冲洗被广泛用作鼻窦疾病的辅助治疗。本研究旨在评估与单纯生理盐水冲洗相比，链霉素或臭氧鼻腔冲洗是否能提供额外获益。
方法： 我们使用22只雌性Sprague-Dawley大鼠诱导细菌性鼻窦炎。第1组不进行冲洗，第2组用生理盐水冲洗，第3组用链霉素冲洗，第4组用臭氧溶液冲洗。治疗后，使用微生物学方法评估窦黏膜样本以测量细菌载量。测定组织病理学炎症率，并使用扫描电子显微镜半定量评估生物膜形成。
结果： 冲洗后，所有组均观察到细菌计数显著减少。在链霉素组中，管腔内的活菌大部分减少；然而，黏附于黏膜的细菌仍然存在。尽管链霉素组在生物膜和炎症方面均显示出最低的平均评分，但这些差异无统计学意义。总体而言，各组之间无统计学显著差异。
结论： 本实验研究探讨了急性细菌性鼻窦炎模型中的生物膜形成。冲洗操作与细菌载量减少相关，但在组织病理学表现或生物膜形成方面，各组之间未观察到统计学显著差异。这些发现应谨慎解读，需要更大样本量的进一步研究来阐明其潜在的临床意义。
证据级别： 不适用（临床前动物模型）。
英文摘要
OBJECTIVE: Rhinosinusitis is characterized by inflammation of the nasal mucosa and paranasal sinuses. Nasal irrigation is widely used as an adjunctive treatment for sinonasal diseases. The aim of this study was to evaluate whether nasal irrigation with streptomycin or ozone provides additional benefit compared with saline irrigation alone.
METHODS: We used 22 female Sprague-Dawley rats to induce bacterial rhinosinusitis. Group 1 was not irrigated, group 2 was irrigated with saline, group 3 with streptomycin, and group 4 with ozone solution. After the treatment, sinus mucosa samples were evaluated using microbiological methods to measure bacterial load. The histopathological inflammation rate was determined, and biofilm formation was assessed semi-quantitatively using scanning electron microscopy.
RESULTS: After irrigation, a significant reduction in bacterial counts was observed in all groups. In the streptomycin group, viable bacteria in the lumen were largely reduced; however, bacteria that adhered to the mucosa remained. Although the streptomycin group showed the lowest average scores for both biofilm and inflammation, these differences were not statistically significant. Overall, there was no statistically significant difference between the groups.
CONCLUSIONS: This experimental study explored biofilm formation in an acute bacterial rhinosinusitis model. Irrigation procedures were associated with a reduction in bacterial load, but no statistically significant differences were observed between groups in terms of histopathological findings or biofilm formation. These findings should be interpreted cautiously, and further studies with larger sample sizes are required to clarify their potential clinical relevance.
LEVELS OF EVIDENCE: Not applicable (preclinical animal model).