声带损伤后黄斑细胞增殖的检测
Detection of macula flava cells proliferation after vocal fold injury.
文献信息
| PMID | 42826530 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Xumao Li |
| 作者单位 | Huashan Hospital Fudan University, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China. |
| 期刊 | Brazilian journal of otorhinolaryngology |
| SCI 分区 | Q2 |
| IF | 2.1 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 咽喉科 |
中文摘要
目的： 黄斑细胞在声带损伤修复中的作用仍不清楚。本研究旨在确定它们在声带损伤后是否增殖。
方法： 将20只雄性Sprague-Dawley大鼠分为对照组（n=5）和实验组（n=15）。实验组大鼠的左声带受到损伤。大鼠在术后第1、3和7天被安乐死。使用苏木精和伊红（HE）染色检查愈合过程。使用多色免疫荧光检测细胞类型：增殖细胞（5-乙炔基-2'-脱氧尿苷，EdU）、黄斑细胞（胶质纤维酸性蛋白，GFAP）和成纤维细胞（波形蛋白）。
结果： 损伤后一天，受损声带缺乏上皮层并表现出组织水肿。损伤后三天，上皮细胞已开始覆盖受损区域。到损伤后七天，受损区域完全被上皮细胞覆盖并显示萎缩。固有层中EdU阳性成纤维细胞在第3天显著增加，随后在第7天明显减少（p<0.05）。受损声带的黄斑细胞在第3天和第7天增殖（p=0.008）。
结论： 声带损伤在七天内得到修复。结果表明，在声带损伤修复过程中，黄斑细胞和成纤维细胞的增殖行为不同。黄斑细胞也可能参与损伤修复。
英文摘要
OBJECTIVES: The role of macula flava cells in vocal fold injury repair remains unknown. This study aimed to determine whether they proliferate following vocal fold injury.
METHODS: Twenty male Sprague-Dawley rats were divided into control group (n = 5) and experimental group (n = 15). The left vocal fold was injured in rats of the experimental group. The rats were euthanized on postoperative day 1, 3, and 7. Hematoxylin and Eosin (HE) staining was used to examine healing process. Multicolor immunofluorescence was used to detect cell types: proliferating cells (5-Ethynyl-2'-deoxyuridine, EdU), macula flava cells (Glial Fibrillary Acidic Protein, GFAP), and fibroblasts (vimentin).
RESULTS: One day post-injury, the injured vocal fold lacked an epithelial layer and exhibited tissue edema. Three-day post-injury, epithelial cells had begun to cover the injured region. By seven-day post-injury, the injured region fully covered by epithelial cells and displayed atrophy. EdU-positive fibroblasts in the lamina propria significantly increased at day 3, followed by a pronounced decrease at day 7 (p < 0.05). The macula flava cells of injured vocal fold proliferated at day 3 and 7 (p = 0.008).
CONCLUSION: Vocal fold injuries were repaired within seven days. The results indicated that proliferate behavior differs between macula flava cells and fibroblasts during vocal fold injury repair. Macula flava cells may be also involved in the injury repair.