疾病修饰疗法通过NK细胞-微生物群相互作用与多发性硬化症中嗅觉功能改善相关。
Disease-modifying therapies are associated with improved olfactory function via NK cell- microbiota interactions in multiple sclerosis.
文献信息
| PMID | 42807502 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Zhuoma Danzhen |
| 作者单位 | Department of Neurology, Affiliated Hospital of Guizhou Medical University, Guizhou Medical University, Guiyang, China. |
| 期刊 | Frontiers in immunology |
| SCI 分区 | Q1 |
| IF | 7.4 |
| 研究类型 | 临床研究 · 临床 |
| 所属专科 | 鼻科 |
中文摘要
目的： 探讨多发性硬化症（MS）中鼻腔微生物群与疾病修饰疗法（DMTs）、淋巴细胞亚群及嗅觉功能的关联。
方法： 纳入30例MS患者和30例年龄、性别匹配的健康对照（HCs）。患者分为DMT治疗组（MS[DMT+]）和未治疗组（MS[DMT-]）。使用宾夕法尼亚大学嗅觉识别测试（UPSIT）评估嗅觉功能。通过流式细胞术测量淋巴细胞亚群，并通过16S rRNA测序分析鼻腔微生物群。
结果： MS患者的嗅觉功能低于HCs，且MS(DMTs+)的嗅觉功能优于MS(DMTs-)（p=0.016，95% CI 0.5614至4.941）。MS(DMTs+)的NK细胞计数低于MS(DMTs-)（p=0.049，95% CI -244.7至-0.2109）。并且MS(DMTs+)中的NK细胞计数与UPSIT相关（r=-0.527，p=0.029）。与HCs相比，MS(DMTs-)显示更高的Shannon多样性（p=0.038）、增加的变形菌门（p=0.004）、减少的莫拉菌科（p=0.037）和肠杆菌科（p=0.033），以及富集的支链氨基酸生物合成（p=0.026）。在MS(DMTs+)中，弯曲杆菌属、厌氧球菌属、嗜胨菌属、普雷沃菌属和Finegoldia与循环NK细胞呈正相关（所有p<0.05）。
结论： DMTs可能改善MS中的嗅觉功能，可能通过调节循环NK细胞相关的免疫状态和鼻腔微生物群，以及控制疾病相关炎症来实现。
英文摘要
OBJECTIVE: To examine the associations of nasal microbiota with disease-modifying therapies (DMTs), lymphocyte subsets and olfactory function in multiple sclerosis (MS).
METHODS: Thirty patients with MS and 30 age- and sex-matched healthy controls (HCs) were enrolled. Patients were classified as DMT-treated (MS[DMT+]) or untreated (MS[DMT-]). Olfactory function was assessed using the University of Pennsylvania Smell Identification Test (UPSIT). Lymphocyte subsets were measured by flow cytometry, and nasal microbiota were analysed by 16S rRNA sequencing.
RESULTS: Olfactory function was lower in MS than in HCs, and better in MS(DMTs+) than in MS(DMTs-) (p =0.016, 95% CI 0.5614 to 4.941). NK cell counts were lower in MS(DMTs+) than in MS(DMTs-) (p =0.049, 95% CI -244.7 to -0.2109). And NK cell counts in MS(DMTs+) correlate with UPSIT (r =-0.527, p =0.029). Compared with HCs, MS(DMTs-) showed higher Shannon diversity (p =0.038), increased Proteobacteria (p =0.004), reduced Moraxellaceae (p =0.037) and Enterobacteriaceae (p =0.033), and enriched branched-chain amino acid biosynthesis (p =0.026). In MS(DMTs+), Campylobacter, Anaerococcus, Peptoniphilus, Prevotella, and Finegoldia were positively correlated with circulating NK cells (all p<0.05).
CONCLUSION: DMTs may improve olfactory function in MS, possibly through modulation of circulating NK cell-associated immune status and nasal microbiota, in addition to controlling disease-related inflammation.