衰老大鼠模型中声带弓形与甲杓肌肌细胞尺寸的相关性研究
Correlating Vocal Fold Bowing and Thyroarytenoid Myocyte Dimensions in an Aging Rat Model.
文献信息
| PMID | 42788716 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Carson S Miller |
| 作者单位 | Division of Laryngology, Department of Otolaryngology - Head and Neck Surgery, University of Washington School of Medicine, Seattle, Washington, USA. |
| 期刊 | The Laryngoscope |
| SCI 分区 | Q2 |
| IF | 2.3 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 咽喉科 |
中文摘要
背景： 喉肌萎缩被认为与年龄相关性声带萎缩（ARVA）中观察到的声带变化有关。尽管大鼠模型被用于研究喉部衰老，但尚不清楚大鼠是否经历类似的年龄相关变化。主要目的是通过内镜量化衰老大鼠的声带萎缩，次要目的是检查喉部组织学并将肌纤维形态计量学与内镜发现相关联。
方法： 使用4、18和24月龄的Sprague-Dawley大鼠（n=24）。收集声带的内镜视频。弓形指数（BI）由两名评估者独立测量。分析甲杓肌以计算肌纤维数量、最小Feret直径（MFD）和横截面积（CSA）。
结果： 18月和24月龄大鼠的BI大于4月龄大鼠（平均差7.15，95% CI：4.39-9.90；平均差4.26，95% CI：1.50-7.01）。平均MFD在4月龄为19.8±1.3μm，18月龄为22.1±1.7μm，24月龄为22.5±1.5μm（4 vs. 18，p=0.014；4 vs. 24，p=0.005；18 vs. 24，p=0.87）。平均CSA在4、18和24月龄分别为435.8±49.4μm²、535.2±81.3μm²和556.6±54.3μm²（4 vs. 18，p=0.013；4 vs. 24，p=0.003；18 vs. 24，p=0.780）。
结论： 衰老大鼠表现出声带弓形增加，与ARVA临床发现相似。衰老大鼠还表现出肌纤维尺寸增加。未来研究将探讨声带弓形伴随肌纤维尺寸增加的驱动机制，包括肌纤维类型转变、代偿性肥大和线粒体功能改变。
证据等级： 不适用。
英文摘要
BACKGROUND: Laryngeal muscle atrophy is hypothesized to contribute to vocal fold changes observed in age-related vocal atrophy (ARVA). Although rat models are used to study laryngeal aging, it is unclear whether rats experience similar age-related changes. The primary aim was to quantify vocal fold atrophy endoscopically in aging rats, with a secondary aim to examine laryngeal histology and correlate muscle fiber morphometry with endoscopic findings.
METHODS: 4-, 18-, and 24-month-old Sprague-Dawley rats were used (n = 24). Endoscopic video of the vocal folds was collected. Bowing Index (BI) was independently measured by two reviewers. Thyroarytenoid muscles were analyzed to calculate myofiber number, minimum Feret diameter (MFD), and cross-sectional area (CSA).
RESULTS: BI was greater in 18- and 24-month rats than in 4-month rats (MD 7.15, 95% CI: 4.39-9.90; and MD 4.26, 95% CI: 1.50-7.01, respectively). Mean MFD was 19.8 ± 1.3 μm at 4 months, 22.1 ± 1.7 μm at 18 months, and 22.5 ± 1.5 μm at 24 months (4 vs. 18, p = 0.014; 4 vs. 24, p = 0.005; 18 vs. 24, p = 0.87). Mean CSA was 435.8 ± 49.4 μm2, 535.2 ± 81.3 μm2, and 556.6 ± 54.3 μm2 at 4, 18, and 24 months, respectively (4 vs. 18, p = 0.013; 4 vs. 24, p = 0.003; 18 vs. 24, p = 0.780).
CONCLUSIONS: Aged rats exhibited increased vocal fold bowing, paralleling ARVA clinical findings. Aged rats also demonstrated an increase in myofiber size. Future studies will investigate the mechanisms driving increased myofiber size with vocal fold bowing, including shifts in muscle fiber type, compensatory hypertrophy, and altered mitochondrial function.
LEVEL OF EVIDENCE: NA.