断奶后食物质地对唐氏综合征幼年Ts65Dn小鼠模型吞咽功能的影响
The impact of postweaning food texture on swallow function in the juvenile Ts65Dn mouse model of Down syndrome.
文献信息
| PMID | 42785614 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Gaurav Singhal |
| 作者单位 | Department of Otolaryngology, Head and Neck Surgery, University of Wisconsin, Madison, WI, United States. Electronic address: gsinghal4@wisc.edu. |
| 期刊 | Physiology & behavior |
| SCI 分区 | Q2 |
| IF | 3.1 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 咽喉科 |
中文摘要
背景： 唐氏综合征（DS）儿童常表现出喂养和吞咽困难，包括长期依赖软食。Ts65Dn小鼠模型反映了DS中的口运动挑战，但断奶后延长软食条件的影响尚不明确。我们研究了从出生后第21天（P21）至P34天的软食条件是否会改变Ts65Dn与整倍体对照小鼠的口腔运动行为、体重、吞咽功能以及舌下神经核BDNF表达，假设软食会影响这些结局。
方法： 雄性和雌性Ts65Dn及整倍体同窝仔鼠从P21至P34被分配至硬食或软食（HF、SF）。记录每日体重和饮水量。视频荧光吞咽研究（VFSS）测量吞咽率、吞咽间期（ISI）、下颌运动率（JER）和下颌周期吞咽比。在P35定量舌下神经核中的proBDNF和成熟BDNF。饮水量在笼水平分析，BDNF结局使用广义线性模型分析。数据使用混合效应和三因素ANOVA及Sidak事后校正分析（α = 0.05）。
结果： Ts65Dn在P21和P34的体重显著低于整倍体对照。所有基因型组的小鼠在HF条件下的舔水量显著多于SF条件。VFSS显示基因型对ISI有显著影响，Ts65Dn的ISI高于整倍体小鼠。JER检测到性别×食物质地×基因型交互作用，雌性Ts65Dn HF小鼠的JER低于雌性整倍体HF小鼠。proBDNF、成熟BDNF或成熟BDNF/proBDNF比值的总体模型均未达到统计学显著性，尽管观察到性别与proBDNF以及食物条件与成熟BDNF的预测因子水平关联。
结论： 软食并未加重VFSS测量中基因型特异性差异。预测因子水平的BDNF发现是探索性的，因为相应的总体模型未达到统计学显著性。
英文摘要
BACKGROUND: Children with Down syndrome (DS) often show feeding and swallowing difficulties, including prolonged reliance on soft diets. The Ts65Dn mouse model mirrors oromotor challenges in DS, yet the impacts of extended postweaning periods of soft food conditions are not well understood. We examined whether a soft food condition from postnatal day (P)21-P34 alters oral motor behaviors, weight, swallow function, and hypoglossal nucleus BDNF expression in Ts65Dn vs. euploid controls, hypothesizing that soft food would impact these outcomes.
METHODS: Male and female Ts65Dn and euploid littermates were assigned to hard or soft food (HF, SF) from P21-P34. Daily body weight and water intake were recorded. Videofluoroscopic swallow studies (VFSS) measured swallow rate, inter-swallow interval (ISI), jaw excursion rate (JER), and jaw cycle swallow ratio. ProBDNF and mature BDNF were quantified in hypoglossal nucleus at P35. Water consumption was analyzed at the cage level, and BDNF outcomes were analyzed using generalized linear models. Data were analyzed using mixed effects and three-way ANOVA with Sidak post hoc correction (α = 0.05).
RESULTS: Ts65Dn weighed significantly less than euploid control at P21 and P34. Mice in all genotype groups licked significantly more water in the HF condition than in the SF condition. VFSS revealed a significant genotype effect on ISI, with higher ISI in Ts65Dn than euploid mice. A sex x food texture x genotype interaction was detected for JER, with female Ts65Dn HF mice showing lower JER than female euploid HF mice. None of the overall models for proBDNF, mature BDNF, or the mature BDNF/proBDNF ratio reached statistical significance, although predictor level associations were observed for sex with proBDNF and food condition with mature BDNF.
CONCLUSIONS: Soft diets did not worsen genotype specific differences in VFSS measures. Predictor level BDNF findings were exploratory because the corresponding overall models were not statistically significant.