Article record

约氏乳杆菌N5上清液保护RPMI-2650细胞免受HDM诱导的损伤，其作用与抑制NR4A1-IRE1-XBP1介导的未折叠蛋白反应相关。

Lactobacillus johnsonii N5 Supernatant Protects RPMI-2650 Cells from HDM-Induced Injury in Association with Suppression of the NR4A1-IRE1-XBP1-Mediated Unfolded Protein Response.

基础研究鼻科IF 4.5Q2

文献信息

PMID	42783759
原文	在 PubMed 查看原文 ↗
发表日期	2026
作者	Wei Ma 查看其他 8 位作者 Yufei Chen；Yaxin Feng；Long Yuan；Haoyu Liu；Yangzi Sun；Zixiang Xu；Demin Cai；Haiyong Sun
作者单位	Department of Otolaryngology, Head and Neck Surgery, Northern Jiangsu People's Hospital, Yangzhou 225001, China. 查看其他 5 条单位信息 The Yangzhou Clinical Medical College, Xuzhou Medical University, Yangzhou 225001, China.；Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou 225001, China.；Laboratory of Animal Physiology and Molecular Nutrition, Jiangsu Key Laboratory of Animal Genetics, Breeding and Molecular Design, Yangzhou University, Yangzhou 225009, China.；Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University, Yangzhou 225009, China.；Guangling College, Yangzhou University, Yangzhou 225009, China.
期刊	Metabolites
SCI 分区	Q2
IF	4.5
研究类型	基础研究 · 基础／转化
所属专科	鼻科

中文摘要

背景/目的： 屋尘螨（HDM）是重要的过敏原，可导致鼻上皮细胞损伤，并在过敏性鼻炎的病理生理学中发挥作用。约氏乳杆菌N5上清液（N5sup）是一种益生菌菌株，可能具有保护特性；然而，其涉及的分子过程仍不清楚。方法：将RPMI-2650人鼻上皮癌细胞分别接受HDM、N5sup或两者联合处理。结果：HDM刺激诱导了一定程度的转录改变。与对照组相比，HDM处理的细胞中共鉴定出221个差异表达基因，这些基因主要富集于未折叠蛋白反应（UPR）通路的IRE1-XBP1分支。综合研究表明，HDM增加了NR4A1-IRE1-XBP1信号通路，表现为NR4A1、ERN1（IRE1）和XBP1表达水平升高。qRT-PCR和Western blotting均证实，N5sup强烈抑制了HDM诱导的NR4A1、IRE1和XBP1升高。蛋白质-蛋白质相互作用网络分析进一步证实了一个由NR4A1、IRE1和XBP1组成的中心枢纽。结论：N5sup上清液保护RPMI-2650细胞免受HDM诱导的损伤，这种保护作用与NR4A1-IRE1-XBP1通路相关。这些发现表明，该信号轴可能作为益生菌干预过敏性气道疾病的潜在治疗靶点。

英文摘要

Background/Objectives: House dust mites (HDMs) are significant allergens that cause damage to nasal epithelial cells and have a role in the pathophysiology of allergic rhinitis. Lactobacillus johnsonii N5 supernatant (N5sup), a probiotic strain, exhibits possible protective properties; nevertheless, the molecular processes involved remain unclear. Methods: RPMI-2650 human nasal epithelial carcinoma cells were subjected to treatment with HDM, N5sup, or a combination of both. Results: The HDM stimulation induced a certain degree of transcriptional alterations. Compared with the control group, a total of 221 differentially expressed genes were identified in HDM-treated cells, which were mainly enriched in the IRE1-XBP1 branch of the unfolded protein response (UPR) pathway. Comprehensive investigation demonstrated that HDM increased the NR4A1-IRE1-XBP1 signaling pathway, characterized by elevated expression levels of NR4A1, ERN1 (IRE1), and XBP1. Both qRT-PCR and Western blotting verified that N5sup strongly inhibited the HDM-induced increase in NR4A1, IRE1, and XBP1. Analysis of the protein-protein interaction network further confirmed a central hub comprising NR4A1, IRE1, and XBP1. Conclusions: N5sup supernatant protects RPMI-2650 cells from HDM-induced injury, and this protective effect is associated with the NR4A1-IRE1-XBP1 pathway. These findings suggest that this signaling axis may serve as a potential therapeutic target for probiotic intervention in allergic airway diseases.