胆脂瘤中耳听小骨的三维显微计算机断层扫描分析：形态学见解与临床意义。
Three-dimensional micro-computed tomography analysis of middle ear ossicles in cholesteatoma: morphological insights and clinical implications.
文献信息
| PMID | 42747530 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Milan Urik |
| 作者单位 | Department of Pediatric Otorhinolaryngology, University Hospital Brno, Cernopolni 9, Brno, 61300, Czech Republic. urik.milan@fnbrno.cz. |
| 期刊 | European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery |
| SCI 分区 | Q1 |
| IF | 2.3 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 耳科 |
中文摘要
目的： 本研究旨在重建健康中耳听小骨以及儿童胆脂瘤破坏的听小骨的三维（3D）结构，并比较3D显微CT扫描与组织学发现，重点检测表皮侵入中耳听小骨的情况。
方法： 对标准手术中获取的9块受胆脂瘤影响的中耳听小骨进行分析，并与面神经减压手术中获取的儿童健康听小骨进行比较。使用显微CT扫描和组织化学分析对听小骨结构进行分析，重点检测表皮。
结果： 组织学上，受胆脂瘤影响的听小骨表面偶见表面上皮（柱状纤毛上皮）（CKAE1/3阳性），但未检测到鳞状上皮（p63阴性）。显微CT分析显示受胆脂瘤影响的中耳听小骨结构发生病理改变。
结论： 虽然3D显微CT扫描显示受胆脂瘤影响的中耳听小骨严重损伤，但组织化学分析未检测到鳞状上皮。
英文摘要
OBJECTIVES: This study's aims were to reconstruct three-dimensional (3D) structures of healthy middle ear ossicles and of ossicles destroyed by cholesteatoma in children and to compare the 3D micro-CT scans and histological findings with a focus on detecting epidermal invasion into the middle ear ossicles.
METHODS: Nine middle ear ossicles affected by cholesteatoma obtained during standard surgery were analysed and compared to healthy ossicles harvested from children obtained during facial nerve decompression surgery. The structures of the ossicles were analysed using micro-CT scan and histochemical analysis with a focus on detecting epidermis.
RESULTS: Histologically, the surface epithelium (columnar ciliated epithelium) was sporadically present on the surface of the ossicles affected by cholesteatoma (CKAE1/3 positive), but squamous epithelium was not detected (p63 negative). Micro-CT analysis shows pathological changes in the structure of the middle ear ossicles affected by cholesteatoma.
CONCLUSION: While 3D micro-CT scans show huge damage of the middle ear ossicles affected by cholesteatoma, no squamous epithelium was detected using histochemical analysis.