Mir-146a rs2910164与过敏性鼻炎易感性的关联研究

文献信息

中文摘要

过敏性鼻炎（AR）虽不威胁患者生命，但严重损害其生活质量。微小RNA-146a（miR-146a）在免疫调节和炎症反应中至关重要；然而，miR-146a rs2910164多态性与AR易感性之间的关系仍不完全清楚。本病例对照研究旨在探讨miR-146a rs2910164多态性对AR易感性的影响。本研究共纳入180名非AR志愿者和211名AR患者。采用RT-qPCR检测血浆miR-146a表达；采用TaqMan-qPCR方法检测rs2910164基因型。采用ELISA检测血清IgE、IL-4、IL-6和IL-10水平。采用Pearson相关分析评估miR-146a表达与TNSS评分、RQLQ评分以及血清因子浓度之间的线性相关性。通过logistic回归确定AR的危险因素。miR-146a rs2910164的GG基因型和G等位基因在AR患者中的频率显著高于健康对照。AR患者血浆miR-146a水平降低。携带GG基因型的AR患者血浆miR-146a水平更低，表现出更活跃的过敏和炎症反应。miR-146a表达与过敏症状及临床表现严重程度密切相关。Logistic回归表明，血清IgE和IL-4水平是AR的独立危险因素，IL-10和miR-146a表达是独立保护因素。AR患者血浆miR-146a水平较低，miR-146a rs2910164的GG基因型增加AR易感性。

英文摘要

Allergic rhinitis (AR) does not pose a threat to patients' lives, but it seriously impairs their quality of life. MicroRNA-146a (miR-146a) is critical for immune regulation and inflammatory responses; however, the relationship between the miR-146a rs2910164 polymorphism and susceptibility to AR remains incompletely understood. This case-control study was designed to investigate the influence of the miR-146a rs2910164 polymorphism on susceptibility to AR. This study included a total of 180 non-AR volunteers and 211 AR patients. The expression of plasma miR-146a was measured by RT-qPCR; The rs2910164 genotype was detected using the TaqMan-qPCR method. ELISA was used to detect the levels of serum IgE, IL-4, IL-6, and IL-10. Pearson correlation analysis evaluated the linear correlations between the expression of miR-146a and the TNSS score, RQLQ score, as well as the concentration of serum factors. The risk factors of AR were identified through logistic regression. The GG genotype and G allele of miR-146a rs2910164 were significantly more frequent in AR patients than in healthy controls. The plasma miR-146a level was reduced in AR patients. The plasma miR-146a level was lower in AR patients carrying the GG genotype, exhibiting more active allergic and inflammatory responses. The miR-146a expression was closely related to allergic symptoms and the severity of clinical manifestations. Logistic regression indicates that the levels of serum IgE and IL-4 were independent risk factors for AR, IL-10 and miR-146a expression were independent protective factors. Low plasma miR-146a levels are observed in AR patients, and the GG genotype of miR-146a rs2910164 increases susceptibility to AR.