鸡胚胎前庭I型和II型毛细胞表达离子通道的多组学分析
Multi-omics analysis of ion channels expressed by chicken embryo vestibular type I and type II hair cells.
文献信息
| PMID | 42740711 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Giulia Cheli |
| 作者单位 | Department of Brain and Behavioral Sciences, University of Pavia, Pavia, Italy. |
| 期刊 | Experimental physiology |
| SCI 分区 | Q2 |
| IF | 3.2 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 耳科 |
中文摘要
平衡和凝视依赖于前庭I型和II型毛细胞对头部运动的快速、准确检测和信号传递。信号转导和传递涉及多种类型的离子通道，这些通道在毛细胞分化过程中逐步获得，其身份仅部分已知。在本研究中，我们采用多组学方法，鉴定了鸡前庭毛细胞在胚胎第15天至胚胎第21天（孵化当天）之间表达的离子通道的分子性质，此时I型和II型毛细胞均表现出功能成熟。I型毛细胞的特征是表达一种低电压激活的外向整流K+电流，称为IKL。在成年小鼠中，IKL由KV1.8 α亚基承载，尽管KV7.4 α亚基是否参与未成熟小鼠I型毛细胞的IKL仍有争议。在本研究中，未发现KV7通道特异性阻断剂linopirdine对未成熟或成熟I型或II型毛细胞有任何影响。此外，全器官RT-PCR和转录组分析显示KV7.4 α亚基转录本无表达，而检测到KV1.8亚基的转录本。其I型和II型毛细胞中的表达最终通过膜片钳测序实验得到证实。对单个毛细胞转录组的进一步分析揭示了Na+、K+和Ca2+通道的若干其他α和辅助亚基转录本的存在，这些可能负责鸟类和哺乳动物中记录的离子电流。
英文摘要
Balance and gaze rely on the rapid and accurate detection and signalling of head movements by vestibular type I and type II hair cells. Signal transduction and transmission involve several types of ion channels, which are acquired progressively during hair cell differentiation and whose identity is known only in part. In the present study, we adopted a multi-omics approach to identify the molecular nature of ion channels expressed by chicken vestibular hair cells between embryonic day 15 and embryonic day 21, the day of hatching, when both type I and II hair cells appear functionally mature. Type I hair cells are characterized by the expression of a low-voltage-activated outward rectifying K+ current, termed IKL. In adult mice, IKL is carried by the KV1.8 α-subunit, although it is still debatable whether the KV7.4 α-subunit contributes to IKL in immature mouse type I hair cells. In this study, no effect of linopirdine, a specific blocker of KV7 channels, was found in either immature or mature type I or II hair cells. Furthermore, whole-organ RT-PCR and transcriptome analysis showed no expression of the KV7.4 α-subunit transcript, while detecting the transcript for the KV1.8 subunit. Its expression in type I and II hair cells was finally confirmed by patch-sequencing experiments. Further analysis of the transcriptomes of individual hair cells revealed the presence of transcripts for several other α and auxiliary subunits of Na+, K+ and Ca2+ channels, which are, presumably, responsible for the ionic currents recorded in both birds and mammals.