Article record

2023-2025年越南北部肉鸡病鸡传染性喉气管炎病毒的分子特征分析

Molecular Characterization of Infectious Laryngotracheitis Virus from Broiler Diseased Chickens in Northern Vietnam During 2023-2025.

基础研究咽喉科IF 6.3Q1

文献信息

PMID	42737448
原文	在 PubMed 查看原文 ↗
发表日期	2026
作者	Giang Thi Huong Tran 查看其他 5 位作者 Vuong Trong Tran；Amonpun Rattanasrisomporn；Hieu Van Dong；Autchara Kayan；Jatuporn Rattanasrisomporn
作者单位	Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam. 查看其他 5 条单位信息 Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand.；National Center for Veterinary Diagnosis No. 2, Department of Animal Health and Production, Ministry of Agriculture and Environment, Hanoi 131000, Vietnam.；Interdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand.；Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.；Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
期刊	International journal of molecular sciences
SCI 分区	Q1
IF	6.3
研究类型	基础研究 · 基础／转化
所属专科	咽喉科

中文摘要

基于PCR的监测在越南北部三个省的14个商业肉鸡场中的8个（57.14%）鉴定出传染性喉气管炎病毒（ILTV）基因组。对2023-2025年获得的ILTV分离株的感染细胞蛋白4（ICP4）以及糖蛋白B、G和J（gB、gG和gJ）基因进行部分测序，表明8份越南ILTV阳性样本为田间ILTV毒株。此外，分离株之间观察到高度核苷酸同一性，gB基因范围为99.74-100%，gJ为99.63-100%，gG为99.66-100%，ICP4为99.34-100%。基于四个基因（gB、gJ、gG和ICP4）的系统发育分析显示，除gJ基因外，8株ILTV毒株属于两个不同的谱系。ICP4基因分析显示，5株ILTV毒株（VNUA-ILTV-02、VNUA-ILTV-05至VNUA-ILTV-08）与美国Laryno Vac、Poulvac-ILT和ON-2462242/1987毒株聚类，而其余毒株（VNUA-ILTV-01、VNUA-ILTV-03和VNUA-ILTV-04）则形成一个独立组。此外，在已表征的ILTV毒株的gB、gG和gJ蛋白中观察到若干氨基酸变化。

英文摘要

PCR-based surveillance identified the infectious laryngotracheitis virus (ILTV) genome in 8 out of 14 (57.14%) commercial broiler farms across three Northern Vietnam provinces. Partial sequencing of the infected cell protein 4 (ICP4) and glycoprotein B, G, and J (gB, gG, and gJ) genes from the ILTV isolates obtained during 2023-2025 indicated that eight Vietnamese ILTV-positive samples were field ILTV strains. Additionally, high nucleotide identity was observed among the isolates, with ranges of 99.74-100% for the gB, 99.63-100% for the gJ, 99.66-100% for the gG, and 99.34-100% for the ICP4 genes. Phylogenetic analyses based on four genes (gB, gJ, gG, and ICP4) revealed that the eight ILTV strains belonged to two distinct lineages, except for the gJ gene. Analysis of the ICP4 gene showed that the five ILTV strains (VNUA-ILTV-02, VNUA-ILTV-05 to VNUA-ILTV-08) clustered with the United States Laryno Vac, Poulvac-ILT, and ON-2462242/1987 strains, whereas the remaining strains (VNUA-ILTV-01, VNUA-ILTV-03, and VNUA-ILTV-04) were in a distinct group. Furthermore, several amino acid changes were observed in the gB, gG, and gJ proteins of the characterized ILTV strains.