基于UPLC-QTOF-MS/MS和网络药理学的香菊片血液成分分析

文献信息

中文摘要

基于血清药物化学和网络药理学，探讨香菊片（XJPs）治疗鼻炎和鼻窦炎的药效物质基础及作用机制。采用UPLC-QTOF-MS/MS技术结合Compound Discover 3.0对香菊片的体内外成分进行分析鉴定。获得香菊片的血液成分。通过网络药理学构建蛋白相互作用网络，结合GO功能分析和KEGG富集分析，揭示香菊片治疗鼻炎和鼻窦炎的关键活性成分及潜在机制。在香菊片中共鉴定出423种化合物，其中41种为血液成分，包括没食子酸、绿原酸、咖啡酸、表儿茶素、芦丁、迷迭香酸和蒙花苷。这些活性成分可能通过GAPDH、MAPK3、SRC、AKT1、CASP3和IL6等关键靶点调控PI3K-Akt、MAPK、Ras、Rap1等信号通路。香菊片通过多成分-多靶点-多通路发挥治疗鼻炎和鼻窦炎的作用。本研究为香菊片治疗鼻炎和鼻窦炎的作用机制研究提供了参考，并为开发靶向治疗鼻炎和鼻窦炎的新剂型提供了新思路。

英文摘要

Based on serum pharmacochemistry and network pharmacology, the pharmacodynamic material basis and mechanism of Xiangju tablets (XJPs) in the treatment of rhinitis and sinusitis were discussed. UPLC-QTOF-MS/MS technology combined with Compound Discover 3.0 was used to analyze and identify the in vivo and in vitro components of XJP. The blood components of XJP were obtained. The protein interaction network was constructed by network pharmacology, combined with GO functional analysis and KEGG enrichment analysis to reveal the key active components and potential mechanism of XJP in the treatment of rhinitis and sinusitis. A total of 423 compounds were identified in XJP, of which 41 were blood components, including gallic acid, chlorogenic acid, caffeic acid, epicatechin, rutin, rosmarinic acid, and linarin. These active components may regulate PI3K-Akt, MAPK, Ras, Rap1, and other signaling pathways through key targets such as GAPDH, MAPK3, SRC, AKT1, CASP3, and IL6. XJP exerts the effect of treating rhinitis and sinusitis through multicomponent-multitarget-multipathway. This study provides a reference for the study of the mechanism of XJP in the treatment of rhinitis and sinusitis and provides new ideas for the development of new dosage forms for targeted treatment of rhinitis and sinusitis.