伴和不伴息肉的慢性鼻窦炎患者与健康对照者鼻腔黏液代谢物的比较
Comparison of Nasal Mucus Metabolites in Chronic Rhinosinusitis With and Without Polyps and Healthy Controls.
文献信息
| PMID | 42733625 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Michael F Armstrong |
| 作者单位 | Department of Otolaryngology-Head and Neck Surgery Virginia Commonwealth University Richmond Virginia USA. |
| 期刊 | OTO open |
| SCI 分区 | Q2 |
| IF | 1.7 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 鼻科 |
中文摘要
目的： 识别伴鼻息肉的慢性鼻窦炎（CRSwNP）和不伴鼻息肉的慢性鼻窦炎（CRSsNP）患者与健康对照者鼻腔黏液代谢物的差异。
研究设计： 横断面研究。
研究场所： 单一机构的三级医疗中心。
方法： 根据黏膜外观将患者分组：正常黏膜（对照组[n=5]）、病变黏膜（[CRSsNP][n=6]）或息肉样黏膜（[CRSwNP][n=4]）。使用拭子采集黏液。通过非靶向代谢组学，采用液相色谱-质谱联用（LC-MS）检测所有可观测代谢物，对鼻腔黏液代谢物进行定量。进一步选择与鼻窦疾病相关生化通路中的代谢物进行分析。
结果： 在甘油磷脂通路中，甘油磷酸胆碱和3-磷酸甘油在对照组（C）中显著高于CRSwNP和CRSsNP组（错误发现率[FDR]=0.03，C vs CRSwNP倍数变化[FC]=3.83，C vs CRSsNP FC=3.92；以及FDR=0.02，C vs CRSwNP FC=3.05，C vs CRSsNP FC=3.50）。在鞘脂通路中，C24-OH硫苷脂和二氢神经酰胺在CRSwNP中显著高于CRSsNP和对照组黏液（FDR=0.01，CRSwNP vs C FC=146.75，CRSsNP vs C FC=45.55，CRSwNP vs CRSsNP FC=3.22；以及FDR=0.05，CRSwNP vs C FC=4.40，CRSwNP vs CRSsNP FC=2.72）。
结论： 甘油磷酸胆碱和鞘脂类鼻腔黏液代谢物在CRS亚型患者与对照组之间存在差异。鼻腔黏液代谢物可能有助于区分鼻窦炎的亚型。
英文摘要
OBJECTIVE: To identify differences in nasal mucus metabolites from patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP) and healthy controls.
STUDY DESIGN: Cross-sectional.
SETTING: Single institution tertiary care facility.
METHODS: Patients were assigned to groups based on the appearance of normal mucosa (control [n = 5]), diseased mucosa ([CRSsNP] [n = 6]), or polypoid mucosa ([CRSwNP] [n = 4]), respectively. Mucus was collected on swabs. Nasal mucus metabolites were quantified via untargeted metabolomics using liquid chromatography-mass spectrometry (LC-MS) for detection of all metabolites observable with the method. Metabolites in biochemical pathways implicated in sinonasal disease were further selected for analysis.
RESULTS: In the glycerophospholipid pathway, glycerophosphocholine and glycerol-3-phosphate were significantly elevated in controls (C) compared to the CRSwNP and CRSsNP groups (false discovery rate [FDR] = 0.03, C vs CRSwNP fold change [FC] = 3.83, C vs CRSsNP FC = 3.92 and FDR = 0.02, C vs CRSwNP FC = 3.05, C vs CRSsNP FC = 3.50, respectively). In the sphingolipid pathway, C24-OH sulfatide and dihydroceramide were significantly elevated in CRSwNP compared to CRSsNP and control mucus (FDR = 0.01, CRSwNP vs C FC = 146.75, CRSsNP vs C FC = 45.55, CRSwNP vs CRSsNP FC = 3.22 and FDR = 0.05, CRSwNP vs C FC = 4.40, CRSwNP vs CRSsNP FC = 2.72, respectively).
CONCLUSION: Glycerophosphocholine and sphingolipid nasal mucus metabolites differed between patients with subtypes of CRS and controls. Nasal mucus metabolites may aid in differentiating subtypes of sinusitis.