羊膜用于鼓膜修复：一项实验研究。
Amniotic Membrane for Tympanic Membrane Repair: An Experimental Study.
文献信息
| PMID | 42732620 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Ali Murati |
| 作者单位 | Department of Otorhinolaryngology, Istanbul Medeniyet University Faculty of Medicine, İstanbul, Türkiye. |
| 期刊 | The journal of international advanced otology |
| SCI 分区 | Q3 |
| IF | 1.3 |
| 研究类型 | 基础研究 · 基础／转化 |
| 所属专科 | 耳科 |
中文摘要
背景： 本研究旨在评估使用羊膜修复鼓膜（TM）创伤性穿孔的组织病理学效果。
方法： 将30只Wistar白化大鼠随机分为3组。在全身麻醉下，于每只耳的后象限制作急性TM穿孔。A组接受羊膜（AM）治疗，B组接受载体纸治疗。C组留待自行愈合。所有大鼠均于第21天处死以进行组织病理学评估。
结果： 共有3只大鼠因各种原因被排除在研究之外。组织学评估了鼓膜厚度、残余穿孔的存在、成纤维细胞活性和新生血管形成。A组18只耳中有1只存在持续性穿孔，B组20只耳中有9只，C组16只耳中有7只。A组与B组以及A组与C组之间的穿孔闭合率存在显著差异（分别为P=.0089和.0121）。A组与B组以及A组与C组之间的鼓膜厚度也存在显著差异（分别为P=.0291和.0106）。然而，各组之间在新生血管形成（P=.1094）或成纤维细胞活性（P=.7275）方面未发现显著差异。
结论： 本研究表明，应用AM在闭合率和膜厚度方面显著改善TM的愈合。这些结果表明，AM可能在促进创伤性TM穿孔愈合方面发挥治疗作用。
英文摘要
BACKGROUND: The aim of this study was to evaluate the histopathological effects of using amniotic membranes to heal traumatic perforations of the tympanic membrane (TM).
METHODS: Thirty Wistar albino rats were randomly divided into 3 groups. Under general anesthesia, acute TM perforation was performed in the posterior quadrant of each ear. Group A received amniotic membrane (AM) treatment, while Group B received vector paper treatment. Group C was left to heal spontaneously. All the rats were killed on day 21 for a histopathological evaluation.
RESULTS: In total, 3 rats were excluded from the study for various reasons. Tympanic membrane thickness, the presence of residual perforation, fibroblastic activity, and neovascularization were evaluated histologically. There was persistent perforation in one of the 18 ears in Group A, 9 of the 20 ears in Group B, and 7 of the 16 ears in Group C. Significant differences in perforation closure rates were observed between Groups A and B and Groups A and C (P=.0089 and .0121, respectively). Tympanic membrane thickness also differed significantly between Groups A and B and Groups A and C (P=.0291 and .0106, respectively). However, no significant differences were found among the groups regarding neovascularization (P=.1094) or fibroblastic activity (P=.7275).
CONCLUSION: This study demonstrates that applying an AM significantly improves the healing of the TM in terms of closure rate, and membrane thickness. These results suggest that AM could play a therapeutic role in enhancing the healing of traumatic TM perforations.