重复超重力训练通过背根神经节重塑促进前庭代偿

文献信息

中文摘要

前庭代偿依赖于感觉重加权，但重复超重力训练（HT）是否促进双侧前庭损伤（BVL）后的恢复仍不清楚。我们研究了HT对BVL后小鼠运动功能和背根神经节（DRG）转录组的影响。小鼠接受假手术或BVL，并被分配到HT（2 G，60分钟/天，共10天）或无HT（NHT）组。评估体重、翻正反射和转棒性能，并在第7天对L5 DRG进行RNA测序。HT未改善体重下降或前庭依赖性翻正反射缺陷。然而，与NHT相比，HT在第7天导致显著更好的转棒性能。RNA测序鉴定出397个候选基因，富集于肌肉相关和髓鞘形成相关的生物学过程。这些发现表明，重复HT通过促进DRG中的转录变化和增强外周躯体感觉可塑性来促进前庭代偿，而非恢复前庭功能本身。

英文摘要

Vestibular compensation relies on sensory reweighting, but whether repeated hypergravity training (HT) facilitates recovery after bilateral vestibular lesion (BVL) remains unclear. We investigated the effects of HT on motor function and dorsal root ganglion (DRG) transcriptomes in mice following BVL. Mice underwent sham surgery or BVL and were assigned to either HT (2 G, 60 min/day for 10 days) or no HT (NHT). Body weight, righting reflex, and rotarod performance were evaluated, and RNA sequencing of L5 DRGs was performed on day 7. HT did not improve body weight loss or vestibular-dependent righting reflex deficits. However, HT resulted in significantly better rotarod performance on Day 7 compared with NHT. RNA sequencing identified 397 candidate genes, with enrichment of muscle-related and myelination-related biological processes. These findings suggest that repeated HT facilitates vestibular compensation by promoting transcriptional changes in DRGs and enhancing peripheral somatosensory plasticity rather than restoring vestibular function itself.