使用OpenSABR（开源听觉脑干反应系统）评估小鼠听力和听力损失

文献信息

中文摘要

听觉脑干反应（ABR）是一种对声音的神经生理反应的测量方法，广泛应用于临床和研究环境。在此，我们描述OpenSABR（“开源ABR系统”），这是一个用于测量ABR的开源系统，比闭源替代方案更灵活且便宜约五十倍。我们系统的核心是市售的德州仪器ADS1299芯片，用于多通道差分记录。我们设计了辅助硬件和软件来记录和可视化ABR。为了展示高质量记录，我们测量了小鼠对左侧和右侧自由声场听觉咔嗒声的反应。我们表明OpenSABR产生的数据与先前工作一致，引入了分析ABR波形的新方法，将这些方法与现有工具进行基准比较，比较个体内和个体间的变异性，并检测与传导性听力损失相关的听觉敏感性下降。与专有替代方案不同，我们的系统高度灵活，可以定制用于记录其他生物信号，如肌电图（EMG）、心电图（ECG）以及感觉或运动诱发电位。OpenSABR的低成本（约400美元）可以拓宽研究和教育中生物信号记录的可及性。该系统旨在用于研究目的，未经过评估或批准用于人类临床，但如果得到验证，其低成本可以拓宽听力和平衡障碍诊断的可及性。

英文摘要

The auditory brainstem response (ABR) is a measure of the neurophysiological response to sound, widely used in clinical and research settings. Here, we describe OpenSABR ("Open-source System for ABR"), an open-source system for measuring the ABR that is more flexible and about 50 times cheaper than closed-source alternatives. The heart of our system is the commercially available Texas Instruments ADS1299 chip for multichannel differential recording. We designed auxiliary hardware and software to record and visualize the ABR. To demonstrate high-quality recording, we measured the response in mice to free-field auditory clicks from the left and right. We show that OpenSABR yields data consistent with prior work, introduce new methods for analyzing ABR waveforms, benchmark these methods against existing tools, compare variability within and between individuals, and detect the decreased auditory sensitivity associated with conductive hearing loss. Unlike proprietary alternatives, our system is highly flexible and could be customized for recording other biosignals like electromyography (EMG), electrocardiography (ECG), and sensory or motor evoked potentials. The low cost of OpenSABR (about $400) could broaden access to biosignal recording in research and education. This system is intended for research purposes and is not evaluated or approved for clinical use in humans, but if validated its low cost could broaden access to diagnosis of hearing and balance disorders.NEW & NOTEWORTHY We present Open-source System for ABR (OpenSABR), a new and open-source system for recording the auditory brainstem response (ABR) that costs about 50-fold less than some commercial alternatives. All hardware designs, software, and data are freely available. We demonstrate high-quality recordings in mice and new methods for quantifying the ABR, and we show that OpenSABR can detect threshold changes after hearing loss.