嗜酸性粒细胞活化试验检测的CD63表达升高与sIgE水平之间的相关性分析
Correlation Analysis Between Increased CD63 Expression Detected by Eosinophil Activation Test and sIgE Levels.
文献信息
| PMID | 42713623 |
|---|---|
| 原文 | 在 PubMed 查看原文 ↗ |
| 发表日期 | 2026 |
| 作者 | Qianyun Li |
| 作者单位 | Department of Otorhinolaryngology and Head & Neck Surgery, Changping Hospital Affiliated to Beijing University of Chinese Medicine, Beijing, China. |
| 期刊 | Scandinavian journal of immunology |
| SCI 分区 | Q3 |
| IF | 3 |
| 研究类型 | 临床研究 · 临床 |
| 所属专科 | 鼻科 |
中文摘要
本研究通过监测外周血嗜酸性粒细胞在过敏原刺激前后CD63和FcεRI的表达，评估嗜酸性粒细胞活化试验在过敏性鼻炎（AR）中的诊断价值。我们纳入了59例AR患者、35例非过敏性鼻炎（NAR）患者和19例健康对照（HC），并根据sIgE结果和临床症状进行分层。通过流式细胞术将CCR3+细胞鉴定为嗜酸性粒细胞。我们量化了基线嗜酸性粒细胞计数及CD63/FcεRI表达，然后测量了在0.1和1.0 μg/mL尘螨过敏原刺激后的变化。以sIgE为参考标准，使用ROC分析和Spearman相关性评估诊断准确性。AR患者的基线嗜酸性粒细胞计数和CD63表达高于NAR或HC（p < 0.05）。与HC相比，AR和NAR中FcεRI均升高。过敏原刺激后，CD63仅在AR中显著增加，且这种增加呈剂量依赖性（p < 0.05）；FcεRI在任何组中均无反应。在这个探索性队列中，CD63显示出有希望的区分能力，AUC为0.899（0.1 μg/mL）和0.994（1.0 μg/mL）；还观察到与sIgE呈中度正相关（r = 0.358-0.461）。相比之下，FcεRI的表现接近随机水平（AUC 0.534-0.544）。嗜酸性粒细胞CD63活化试验可以检测AR中的过敏原特异性反应，并与sIgE呈中度正相关，提示其作为AR诊断补充工具的潜力。
英文摘要
This study evaluated the diagnostic utility of eosinophil activation testing in allergic rhinitis (AR) by monitoring CD63 and FcεRI expression on peripheral blood eosinophils before and after allergen stimulation. We enrolled 59 AR patients, 35 non-allergic rhinitis (NAR) patients, and 19 healthy controls (HC), stratified by sIgE results and clinical symptoms. Eosinophils were identified via flow cytometry as CCR3+ cells. We quantified baseline eosinophil counts and CD63/FcεRI expression, and then measured their changes following stimulation with 0.1 and 1.0 μg/mL dust mite allergen. Diagnostic accuracy was assessed using ROC analysis and Spearman's correlation with sIgE as the reference standard. AR patients had higher baseline eosinophil counts and CD63 expression than NAR or HC (p < 0.05). FcεRI was elevated in both AR and NAR relative to HC. Upon allergen stimulation, CD63 increased significantly only in AR, and this increase was dose-dependent (p < 0.05); FcεRI showed no response in any group. In this exploratory cohort, CD63 showed promising discriminatory ability, with an AUC of 0.899 (0.1 μg/mL) and 0.994 (1.0 μg/mL); a moderate positive correlation with sIgE was also observed (r = 0.358-0.461). In contrast, FcεRI performed around chance level (AUC 0.534-0.544). The eosinophil CD63 activation test can detect allergen-specific responses in AR and shows a moderate positive correlation with sIgE, suggesting potential as a complementary tool for AR diagnosis.